| Name | Description | Points | Trophies |
| The Birth of an Assassin | Be reborn as Ezio Auditore Da Firenze. 作为Ezio Auditore Da Firenze重生 | 20 | Bronze |
| Arrivederci Abstergo | Break out of Abstergo. 逃出Abstergo | 20 | Bronze |
| Myth Maker | Find the 8 statuettes in Monteriggioni. 在Monteriggioni里找到8个小雕像 | 5 | Bronze |
| Vitruvian Man | Unlock all 20 pieces of Subject 16 s video. 解锁目标16的20段影片 | 20 | Bronze |
| Street Cleaner | Hide 5 dead bodies in a Bale of Hay. 在Bale of Hay藏起5具尸体 | 10 | Bronze |
| Fly Swatter | Kick a Guard while using the Flying Machine. 用飞行器的时候踢到一个守卫 | 5 | Bronze |
| Messer Sandman | Stun 4 guards at once by throwing sand in their face. 用沙子同时迷住4个守卫的眼 | 10 | Bronze |
| Doctor | Perform an Air Assassination on a Poisoned NPC. 空中刺杀一个中毒的NPC | 20 | Bronze |
| No-hitter | Kill 10 enemies while remaining in conflict without being hit. 杀掉10个敌人，但自己不被击中 | 20 | Bronze |
| Kleptomaniac | Pickpocket 1000 Florins. 偷1000个金币 | 10 | Bronze |
| Lightning Strike | Sprint for 100 meters. 冲刺跑100米 | 10 | Bronze |
| Sweeper | Sweep 5 guards at once by using a Long Weapon. 用长柄武器同时扫倒5个守卫 | 10 | Bronze |
| Venetian Gladiator | Discover the Assassins Tomb inside Santa Maria della Visitazione. 在Santa Maria della Visitazione里找到刺客冢 | 20 | Bronze |
| I can see your house from here! | Discover the Assassins Tomb inside the Torre Grossa. 在Torre Grossa里找到刺客冢 | 20 | Bronze |
| Hallowed be thy name | Discover the Assassins Tomb inside the Basilica di San Marco. 在Basilica di San Marco里找到刺客冢 | 20 | Bronze |
| Prison Escape | Discover the Assassins Tomb inside the Rocca di Ravaldino fortress. 在Rocca di Ravaldino fortress里找到刺客冢 | 20 | Bronze |
| Choir Boy | Discover the Assassins Tomb inside Santa Maria del Fiore (The Duomo). 在Santa Maria del Fiore里找到刺客冢 | 20 | Bronze |
| Assassin For Hire | Complete your first assassination mission for Lorenzo Il Magnifico. 为Lorenzo Il Magnifico完成第一个暗杀任务 | 10 | Bronze |
| Macho Man | Defend a womans honor. 捍卫一位女士的荣誉 | 10 | Bronze |
| Steal Home | Win a race against thieves! 赢一场和盗贼的赛跑 | 10 | Bronze |
| Show your Colors | Wear the Auditore cape in each city. 在每个城市都穿上Auditore cape | 10 | Bronze |
| Handy Man | Upgrade a building in the Stronghold. 在城堡里升级一幢建筑 | 10 | Bronze |
| I like the view | Synchronize 10 View Points. 同步10个观察点 | 10 | Bronze |
| High Dive | Perform a Leap of Faith from the Top of Florences Giottos Campanile. 在佛罗伦萨的Giotto钟楼的顶部进行一次信仰跳跃 | 10 | Bronze |
| Mailman | Intercept a Borgia Courrier. 截取一个Borgia家的信使 | 10 | Bronze |
| Tip of the Iceberg | Use your Eagle Vision to scan a Glyph in the environment. 用鹰眼扫描一次周围环境里的噪音 | 10 | Bronze |
| A Piece of the Puzzle | Unlock a piece of Subject 16s video. 解锁目标16的一段影片 | 10 | Bronze |
| Art Connoisseur | Buy a Painting from Florence and Venice. 在佛罗伦萨和威尼斯各买一幅画 | 10 | Bronze |
| Podestà of Monteriggioni | Reach 80% of your strongholds total value. 让你的城堡达到它的80%价值 | 30 | Bronze |
| Perfect Harmony | Tint your clothes with those colors: Wetland Ebony and Wetland Ivory. 把你的衣服染成乌木色和象牙色 | 10 | Bronze |
| In Memory of Petruccio | Collect all the Feathers. 找齐所有羽毛 | 30 | Bronze |
| Red Light Addict | Spend 5000 florins on Courtesans. 在艺妓身上花掉5000金币 | 10 | Bronze |
| Man of the People | Toss more than 300 florins on the ground. 在地上扔300+金币 | 10 | Bronze |
| Victory lies in preparation | Get all Hidden Blades，Item Pouches and Armor upgrades for Ezio. 为主角收集齐所有的武器、道具和防具的升级部件 | 10 | Bronze |
| Welcome to the Animus 2.0 | Enter the Animus 2.0. 进入Animus升级版 | 20 | Silver |
| The Pain of Betrayal | Complete DNA Sequence 1 完成DNA序列1 | 30 | Silver |
| Vengeance | Complete DNA Sequence 2 完成DNA序列2 | 30 | Silver |
| Exit the Son | Complete DNA Sequence 3 完成DNA序列3 | 30 | Silver |
| Bloody Sunday | Complete DNA Sequence 4 完成DNA序列4 | 30 | Silver |
| Undertaker | Discover the Assassins Tomb inside the catacombs under Santa Maria Novella. 在Santa Maria Novella的地下墓穴里找到刺客冢 | 20 | Silver |
| The Conspirators | Complete DNA Sequence 5 完成DNA序列5 | 30 | Silver |
| An Unexpected Journey | Complete DNA Sequence 6 完成DNA序列6 | 30 | Silver |
| Bleeding Effect | Complete training and reenter the Animus. 完成训练关，重新进入Animus | 30 | Silver |
| The Merchant of Venice | Complete DNA Sequence 7 完成DNA序列7 | 30 | Silver |
| The Impenetrable Palazzo | Complete DNA Sequence 8 完成DNA序列8 | 30 | Silver |
| Masquerade | Complete DNA Sequence 9 完成DNA序列 | 30 | Silver |
| Biancas Man | Complete DNA Sequence 10 完成DNA序列10 | 30 | Silver |
| The Prophet | Complete DNA Sequence 11 完成DNA序列11 | 30 | Silver |
| The Vault | Complete DNA Sequence 14 完成DNA序列14 | 30 | Silver |
| An Old Friend Returns | Escape the hideout. 逃出藏身处 | 100 | Gold |
| Master Assassin | Unlock all trophies. | - | Platinum |
